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A morphometric dataset was constructed by measuring/counting 28 characters in 40 species; 17 characters were continuous, and 11 characters were meristic (Table 1; Figure S4).
The number of adult male H. commersoni available in the morphometric dataset was limited, and given there is evidence of sexual dimorphism in this species [ 16, 44], males were excluded from the morphometrics analyses.
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The genetic and geometric morphometric datasets are broadly congruent regarding the biogeographical history of the taxon, albeit with some differences.
Each dataset was independently processed.
Since the first principal component of the morphometric dataset proofed to be mainly influenced by the standard length, it was not used for analysis.
This dataset is termed Erlangen.
While FST was not estimated from the same individuals from which sperm measurements were taken, both the sperm morphometric and microsatellite datasets were derived from the same geographic populations.
Principal component analysis (PCA; SAS Procedure PRINCOMP SASS version 9.1.3; SAS Institute, Inc., Cary, NC) was carried out on the morphometric dataset of 40 species using the 28 measured/counted characters to confirm the correlations of species-groups and to determine the main components of variation in the morphological data.
To infer whether the morphological relationships are correlated with the genetic relationships, we compared the morphometric dataset with a phylogeny reconstructed from the combined dataset of three mtDNA and one nuclear DNA regions.
Quadratic discriminant function analysis (DFA) correctly assigns 72% of samples using the morphometric dataset and just 64% with meristic characters.
The datasets are pretty limited.
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