Homozygous Rtn4r mutant mice were viable and fertile and have normal brain morphology by gross morphological inspection, as well as finer morphometric calculations of cell densities, laminal thickness and the thickness of the corpus callosum (see Text S1 and Fig. S1).
For morphometric calculation, two dimensional measurement techniques were developed after reviewing techniques used in a previously published report by Egbor et al., (2006) [ 9].
Briefly, morphometric quantification involved calculation of the percentage of tissue area that was positively stained within a 40 µm-thick area from the basement membrane extending into the airway lumen.
In this framework we investigated the geomorphic responses to tectonic forcing by the calculation of morphometric parameters, focusing on topography (map of local relief, swath profiles) and hydrography (basin hypsometric curve and integral, basin asymmetry factor, river longitudinal profiles and relative indices), and using SRTM DEM.
Remote Sensing and Geographical Information System (GIS) has been used for the delineation and calculation of the morphometric parameters of the watershed.
For the calculation of the morphometric parameters, the images were segmented using a uniform threshold as described in a previously published paper [ 6].
A. L. Kovács in his paper entitled " A simple method to estimate the number of autophagic elements by electron microscopic morphometry in real cellular dimensions" describes a morphometric method (Ssp method) for calculation of surface values and estimation of average diameter and number of autophagic elements in real cellular dimensions using data from electron micrographs.
The hydrological module in ArcGIS was used for calculation of watershed and morphometric parameters, under linear, relief and aerial aspects.
Currently, the direct calculation of the measurements of morphometric quantitative area shapes has been made possible by utilizing various programs after the common usage of MRG.
Articulated structures such as jaws present a problem to geometric morphometric techniques because movement of one body part relative to another introduces arbitrary variation into the calculation of shape variables.
