Statistical analysis for the morphometric assay was performed using a Wilcoxon, non-parametric rank-sum test.
For the morphometric assay, 25 cells from each condition in 3 different fields were included in the analysis.
One wound specimen from each animal was fixed, stained and used for the morphometric assay of epithelialization rate, whereas another one was freshly frozen and stored at -80°C until use in biochemical assays.
We investigated the effect of E2 on uNK-mediated angiogenesis using an established in vitro assay (Greaves et al., 2013) of endothelial network formation which is considered a morphometric assay that models the reorganization stage of angiogenesis.
From a morphometric assay conducted on 5+5 rats, 75%CI liver resulted represented by more empty zone (index of edema) as compared to 25%CI liver (18.17 ± 5.02 vs 11.89 ± 2.69, respectively; P < 0.001).
Based upon initial observation that the leaflets of 4x plants (as confirmed by nuclear DNA content measurement) looked broader than those of 2x plants, a quantitative morphometric assay was devised, as depicted in Figure 2.
Samples of intestine were obtained for morphometric analysis, myeloperoxidase (MPO, TBARS and GSHH assay.
Percentages of drug-induced cell death and LD90 doses were determined by the use of a standardized morphometric test, the DiSC assay [ 17, 18].
Where we think this report has general implications for many scientists is in the method that you have used, namely to use an inducible-intersectional genetic strategy to label a subset of cells, and to develop the morphometric and quantitative assays for categorizing what you saw.
Cellular proliferation and the mesangial matrix in glomeruli were assayed via histological and morphometric procedures.
