Untreated cells have flattened morphology with condense nucleolus in the nucleus and numerous mitochondria in the cell cytoplasm.
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RING also deals with condensed rings.
The third cell type expressed a high level of mCherry with condensed GFP.
This synergistic induction of cell death was confirmed by increased numbers of cells with typical apoptotic morphology, that is, condensed cytoplasm and nuclei, within 6 h after treatment with TRAIL and paracetamol.
The plates were then sealed and heated with condensing steam to the desired pretreatment temperature.
Mitochondria from DMSO-treated HCT116 cells were of normal morphology, and showed condensed and electron-dense dark cristae; however, after incubation with Os, mitochondria appeared swollen with expanded and transparent matrices.
Cells treated with hsp90 inhibitors exhibit pleiotropic changes, including an expansion of the mitochondrial compartment, accompanied by mitochondrial fragmentation and condensed mitochondrial morphology, with ultimate compromise of mitochondrial integrity and apoptosis.
Non-staining apoptotic cells were recognised in the midst of cells with normal morphology by having either condensed, irregular nuclei frequently with a crescent-shaped appearance or fragmented nuclei within cells showing cytoplasmic withdrawal.
