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Changes in cell morphology were scored manually employing the following criteria: Cells that had a fully-rounded cell body with no membrane extension processes were given one point.
These follicles with normal morphology were scored as healthy follicles.
Microsatellite peaks above the threshold of 100 relative fluorescent units exhibiting characteristic morphology were scored.
All survival (by strip counting) or death assays (apoptotic nuclear morphology) were scored blindly as described previously 16.
Fluorescent signals in at least 30 non-overlapping interphase nuclei with intact morphology were scored using a Zeiss Axioplan 2 microscope (Carl Zeiss Meditec, Inc., Dublin, CA, USA) with a × 100 planar objective, using a triple band-pass filter that permits simultaneous blue, green, and red colours.
Fluorescent signals in at least 30 nonoverlapping interphase nuclei with intact morphology were scored using a Zeiss Axioplan 2 microscope (Carl Zeiss Meditec, Inc., Dublin, CA) with a 100× planar objective, using a triple band-pass filter that permits simultaneous blue, green, and red colors.
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The severity of morphological disruption morphology was scored according to an arbitrary index with 0 being normal and 4 being severely disrupted (Table 1).
Mitochondrial morphology was scored as follows: Normal, a mixed population of interconnected and non-connected, long and short tubular mitochondria; Hyperfused: mitochondria were elongated and highly interconnected, with few non-connected mitochondria; Fragmented, the majority of mitochondria were non-connected, small and spherical.
Hair subtypes were analyzed with more than 400 hairs for each mouse, and their morphology was scored under a dissection microscope.
The morphology was scored concerning proliferation, presence of vacuoles and epitheloid phenotype (Table 4).
Breast lesion morphology was scored according to the sonographic BI-RADS lexicon criteria and classified accordingly.
More suggestions(15)
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