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Colonies displaying a clear stem cell morphology were picked and transferred onto irradiated MEFs or Matrigel-coated plates for further passage.
Colonies with green or blue-green pigmentation (either single celled, filamentous or of diatom morphology) were picked off, diluted and re-plated on filter paper as described above, as well as on BBM pH 6.5 and BG-11 pH 7.1 agar plates (1% agar).
Colonies with iPSC morphology were picked and expanded.
Visibly different colonies based on morphology were picked and streaked on nutrient agar for taxonomic identification.
Colonies with stem cell-like morphology were picked up, dissociated with 0.05% collagenase type IV and dissociated mechanically, and transferred to a feeder layer for expansion.
Approximately 3 to 4 weeks post-infection, newly formed colonies with ESC-like morphology were picked.
Similar(50)
One colony for each observed morphology was picked up, stored in Cystine Triptose Agar (Difco) and sent to the reference microbiology laboratory in Italy, where they were checked for purity and stored at −70°C in glycerol 20% until examined.
For each fermented bran, between 5 and 20 colonies representing all morphologies were picked from the respective plates at the refreshment steps 1, 7 and 12, purified by successive streaking, and stored in glycerol at − 80 °C for further experimentations.
Colonies with different morphologies were picked up, microscopically examined, and purified on plates.
Colonies with different morphologies were picked and pure cultures of these colonies were obtained after three transfers.
Twenty-five days post infection, colonies with hESC-like morphology emerged and were picked up to generate stable cell lines.
More suggestions(15)
morphology were correlated
morphology were tested
morphology were determined
morphology were seen
morphology were followed
morphology were recorded
morphology were noticed
morphology were investigated
morphology were compared
morphology were assessed
morphology were obtained
morphology was picked
morphology were evaluated
morphology were visualized
morphology were isolated
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