Colonies with green or blue-green pigmentation (either single celled, filamentous or of diatom morphology) were picked off, diluted and re-plated on filter paper as described above, as well as on BBM pH 6.5 and BG-11 pH 7.1 agar plates (1% agar).
Colonies with iPSC morphology were picked and expanded.
Visibly different colonies based on morphology were picked and streaked on nutrient agar for taxonomic identification.
Colonies with stem cell-like morphology were picked up, dissociated with 0.05% collagenase type IV and dissociated mechanically, and transferred to a feeder layer for expansion.
Approximately 3 to 4 weeks post-infection, newly formed colonies with ESC-like morphology were picked.
From each RS five colonies with typical E. faecium morphology were picked.
From days 13 to 20 colonies with mink ES cell-like morphology were picked up and expanded.
Up to three suspect colonies based on colony morphology were picked and plated to blood agar plates.
Subsequently, two lactose fermenting colonies with typical E. coli morphology were picked and subjected to confirmatory Gram staining, indole, methyl red, Voges-Proskauer, and Simmons Citratee tests.
For each fermented bran, between 5 and 20 colonies representing all morphologies were picked from the respective plates at the refreshment steps 1, 7 and 12, purified by successive streaking, and stored in glycerol at − 80 °C for further experimentations.
