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The two colony morphology variants of ATCC9372 each exhibited distinct mutation profiles indicating that the reference strain is in fact a mixed population of at least two genetically distinct substrains.
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In this paper, we describe the genetic repertoire of P. aeruginosa to generate morphology variants by single loss-of-function mutations.
It should be noted that a diverse range of colony morphology variants has been observed within each of these spatial niche specialist classes.
According to this model, nevus cells are considered a morphological variant of melanocytes that have assumed a morphology that is more epithelioid and less dendritic.
Pools of morphology variants were then tested in an acute murine airway infection model [35], [36] to evaluate their competitive fitness to grow in vivo and to breach epithelial barriers.
Hip morphology variants may influence the development of osteoarthritis (OA) (Ganz et al. 2008).
To mimic dissociative behaviour of isogenic strains in lungs, pools of 25 colony morphology variants were tested for competitive fitness in an acute murine airway infection model.
Colony morphology variants were caused most frequently by the inactivation of metabolic genes.
The colony morphology variants were allocated to eight distinguishable morphotypes all of which are regularly recovered from chronically infected CF lungs.
The 3,500 transposon mutants of the library were assayed for colony morphology variants.
By utilizing the approach of signature tagged mutagenesis (STM) [32] [34], colony morphology variants were identified by screening a STM minitransposon library grown under different culture conditions in vitro.
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