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To observe the morphology, the cells were seeded at a concentration of 1 × 105/ml in a 10-ml petri dish and incubated for 3 days with a media containing GM or GF at a concentration of 0.2 mg/ml.
Based on their high autofluorescence and morphology, the cells were uniformly identified as macrophages.
As macrophages differentiated towards a preadipocyte morphology, the cells expressed less CD14 and more DLK/S-100 (Fig. 6).
To evaluate nuclear morphology, the cells on cover glass were fixed with 4% paraformaldehyde, stained with DAPI (10 µg/ml) in phosphate-buffered saline (PBS) for 10 min, and then observed under fluorescence microscope (Olympus BX61, Olympus optical Co. Ltd. Tokyo, Japan).
Regarding morphology, the cells cultivated in different conditions were similar.
To investigate their morphology, the cells were immunofluorescently stained for β-catenin, phalloidin and α-smooth muscle actin (αSMA).
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As shown in the SEM image, morphology (the cell wall) of the E. coli was badly damaged by the NDs absorbed with the protein lysozyme [32, 33].
Also, the morphology, the cell distribution and the tendency to hexagonallity are studied.
In continuation, the morphology of the nano-fiber, cell viability, morphology of the cells on the nano-fiber and related results are presented.
The morphology of the cells was different".
The staining pattern was analyzed in combination with the morphology of the cells.
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