We include the nucleolus that, similar to the aforementioned bodies, changes its morphology over the cell cycle.
Analysis of cell morphology over 30 s showed that ∼70% of control peptide and untreated cells displayed a defined leading edge comprising lamella and lamellipodium, whereas 50% of the β2-actinin peptide-treated cells had no definite leading edge, and a further 10% showed minimal lamellipodial extension.
Moreover, surface nanotopography has been shown to exert influence over cell adhesion, morphology, proliferation, migration, differentiation, alignment, cytoskeleton organization, and gene expression in many cell types, including human mesenchymal stem cells (hMSCs) [10]; hMSCs are being increasingly used in therapeutic applications for bone, cartilage, and fat transplantation and repair [11].
To assess cell morphology over an exposure time-course, 1,000 cells/well (estimated by a hemocytometer) were seeded in 100 μL/well of culture medium in 96-well culture plates and incubated at 37 °C in a humidified 5% CO2incubator for 24 h to allow the cells to settle and adhere to the wells.
We noticed a striking difference in the actions of ATP on cell morphology over the M1 M2 polarization gradient, and therefore asked whether this may be associated with their actions to inhibit inflammasome activation.
Cryptococcal cell morphology affects host cell interactions and pathogenicity.
Along these lines, we have very recently shown that the self-assembly of block copolymers into thin films can be used as an extremely labile method to precisely position cellular adhesion molecules, at nanometre lateral spacings, to effect control over cell attachment and morphology.
We previously explored the effects of nanoscale surface topography on single-cell- and tissue-level morphology and function with precise control over cell and tissue structure, such as guided migration in response to topographical gradient or self-assembly of anisotropic cardiac monolayers.
Herein, we report three new MDP sequences and examine how the incorporation of bioactive sequences and the modification of the hydrophilic amino acids affect cell morphology and cell number over time.
At 28 days after differentiation, iPDK and EDK cell lines showed similar fibroblast morphology, characterized by cells with elongated and stellate shapes that maintained this morphology over prolonged passage (Figure 1B).
