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The biocompatibility and morphology of neural cells in the optimized scaffold was also evaluated.
In situ regulation of the morphology of neural tissues derived from human pluripotent stem cells using micropatterned, clickable substrates.
Prior to differentiation, hEPI-NCSC had the typical stellate morphology of neural crest stem cells (Fig. 2A), which remained unchanged after pretreatment with SHH and CHIR99021 and subculture (Fig. 2B).
Because the morphology of neural progenitors changes during development, it would be useful if our electroporation procedure were applicable for visualizing the morphology of single cells in the cerebral cortex of developing ferrets.
The principal new finding is that the Lis1 Nde1 complex is critically required for maintaining the morphology of neural progenitors and their lateral contacts with neighboring cells additional to their role in mitotic spindle regulation.
Cells showed typical morphology of neural crest precursor (Supplementary Material, Fig. S2A), stained positive for neural crest marker Nestin (Supplementary Material, Fig. S2C and D) and were negative for glial fibrillary acidic protein staining (Supplementary Material, Fig. S2E and F).
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One of the applications of altering the surface morphology is of neural implants such as the Utah electrode array (UEA) that communicate with single neurons by charge injection induced stimulation or by recording electrical neural signals.
In vitro differentiation of hESC to the neural lineage recapitulates the in vivo development in multiple aspects, including morphology (formation of neural rosette), timing, and gene expression.
Our results indicate that after a specific protocol of chemical induction, adult rat derived MSC display some neuronal characteristics (morphology, expression of neural proteins), a number of which can also be seen in other stressed or damaged cells.
Previous detailed analysis of spinal neural tube closure has documented three 'modes' of closure, characterized by the precise morphology of the neural folds (6).
The failure to initiate neural tube closure in Vangl2 Lp/Lp and Scrib Crc/Crc mutants has been attributed to abnormal morphology of the neural plate, prior to the stage of Closure 1.
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