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Immunohistochemical staining demonstrated that EP2 and EP3 immunoreactivity was localized in NeuN-positive large cells showing the typical morphology of motor neurons in mice.
Further, we performed electron microscopy to examine mitochondrial morphology of motor neurons.
By examining the morphology of motor neurons in the mutants, we found that some VD neuron axons showed defective morphology in several mutants (Figure 1C and Table 2).
However, AGS-499 enhanced the survival of H2O2-challenged differentiated NSC-34 cells that had attained the morphology of motor neurons (Matusica et al, 2008) by 60% (Supporting information Fig 3A and B).
Interestingly, striking differences could be observed when we compared the morphology of motor neurons transduced with WT-HSPB8 with those expressing mutant HSPB8, as judged by SMI32 neurofilament immunostaining.
Neurofilament heavy antibody SMI32 and the specialized morphology of motor neurons [neuronal cells with large pyramidal soma (diameter > 25 µm) which have more than two neurites] were used to discriminate motor neurons from other cells types during quantification of all experiments.
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To examine the numbers and morphology of the motor neurons, we performed cresol violet staining on the lumbar spinal cord sections from non-treated and treated mice (n = 3 per group).
To determine how the heavy-chain sequence maps onto the morphology of the motor domain, we used EM to examine fusion proteins in which GFP and BFP were inserted at seven different locations.
The response to SCI in the G93A-SOD1 spinal cord seems to replicate some of the most crucial gene expression changes described in the affected tissues during the natural development of ALS and to demonstrate the same changes in the morphology of the motor cell population observed in spinal cord from individuals and animal models of the disease in an advanced stage.
8) The change in the dendritic morphology of the immediate firing motor neurons in SOD mice is not explained and might be a cause of synaptic pruning.
To determine if the decreased number of GABAergic synapses in ju89 mutants was due solely to defects in initial axon outgrowth and extension along the nerve cords, we examined the morphology of the dorsal D (DD) motor neurons in more detail.
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