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Live/Dead and DAPI/Actin staining exhibited viable cell and the morphology of actin fibers and nuclei of MC3T3 cells treated with BG-0 and BG-5.
A qualitative assessment of cell viability with Live/Dead technique and visualization of the morphology of actin fibers and nuclei of MC3T3 cells treated with Li-BGs with DAPI/Actin staining techniques showed that addition of Li resulted in an increase in the mean number of DAPI-labeled nuclei which was in reasonable agreement with MTT test.
To obtain insights into the mechanisms involved in the enhancement of cold hardiness, we investigated the expression patterns of genes encoding subunits of chaperonin (CCT) and the morphology of actin, a substrate of CCT, at low temperatures.
Moreover, patient's fibroblasts showed a lower endocytic activity and an altered morphology of actin fibers, which suggest that aberrant protein recycling processes may underlie ALMS pathogenesis.
The thin filaments of the contractile apparatus are anchored at Z-discs and consistent with the ruptured Z-discs in the MenaGT/GTVASP−/− hearts, the morphology of actin filaments in the I-band also appeared disorganized and chaotic.
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The increased cell population in the G1 phase and the flat, enlarged, vacuole-rich morphology devoid of actin stress fibres (Fig 3B) were reminiscent of senescent cells.
We show that depending on the morphology of the actin shell inside the liposome, spreading dynamics is either reminiscent of a bare liposome (in the case of a sparse actin shell) or of a cell (in the case of a continuous actin shell).
The difference in morphology of the actin protrusions was most prominent at the cell-substratum junction.
Ectopic expression of this mCherry-FMN2 mutant completely restored the number and morphology of the actin structures generated by MMS treatment.
To address this point we performed additional analysis on the correlation between the number and morphology of nuclear actin filaments and the number of 53BP1 foci (a proxy for double-strand breaks).
Furthermore, it has been reported that PKC isoforms influence the morphology of the actin cytoskeleton, as well as the activation of metalloproteases MT1-MMP and MMP-2, reported to be involved in cancer cell migration.
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CEO of Professional Science Editing for Scientists @ prosciediting.com