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Diabetic VSMCs exhibited abnormal morphology in cell culture with loss of the normal hill and valley configuration.
For evaluation of cell number and morphology in cell culture experiments, three pristine and modified HDPE and PLLA samples were used for analysis by randomly chosen fields.
HEK-293 and HEK/CD9P-1 exhibitedifferentnt morphology in cell cultured.
IK induced apoptosis in OSCCCs, as identified by a cell-cycle analysis, annexin V-FITC and propidium iodide staining, and the nuclear morphology in cell death.
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Inhibition of protein farnesyltransferase activity by FTIs has been shown to improve abnormalities in nuclear morphology in cells from subjects with HGPS and from animal models of the disease [15], [16], [17], [18], [19], [20], [32].
Next, we compared the impact of DMOG on endothelial cell morphology in cells migrating from spheroids with the effects of DMOG on cells cultured as monolayers.
Although GFP expression had no effect on cell morphology, in cells expressing PKD-GFP, an extensive accumulation of autophagosomes was apparent.
To further corroborate this notion, we examined the effect of MIEF1-V5 expression on mitochondrial morphology in cells depleted of Mfn2 by RNA interference (RNAi).
Mn2+ increased the extent of sheet formation, and decreased the percentage of cells with a simple morphology, in cells expressing the dominant negative R-Ras construct at the two lower laminin concentrations tested (Fig 4).
As mentioned above, a first hypothesis to explain changes in morphology in cells affected by LSDs is that the impairment of the autophagic process induces the accumulation of undegraded and 'old' fragmented mitochondria.
Snail, a key regulator of EMT, downregulates E-cadherin expression, leading to the loss of epithelial morphology in cells undergoing migration during embryonic development as well as tumor progression [ 66– 66].
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