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Drosophila ATL restored ER morphology defects in mammalian cells lacking ATLs, and measurements of nucleotide-dependent dimerization and GTPase activity were comparable for Drosophila ATL and human ATL1.
40 embryos each from the YFP-positive and the YFP-negative embryos were selected for their normal morphology at 48 hpf as heat shock alone caused some overall morphology defects in approximately 10 20% of embryos.
Furthermore, IKKε::myc suppressed the bristle morphology defects in spn-F mutants (Fig. 3C,G,N).
We did not observe obvious dendritic morphology defects in larval ddaC neurons expressing Cp1 via UAS-Cp1 transgene.
However, mutations of pdr-1 decreased the percentage of gonadal morphology defects in the two ced-10 alleles tested.
DOI: http://dx.doi.org/10.7554/eLife.01323.022 10.7554/eLiFigure23 figuregure 3—figure supplement 1. Transmission electron microscopy (TEM) reveals mitochondrial morphology defects in miR-1 null hearts.
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The vulval morphology defect in bh26 animals shares some similarity with that of bh13 animals.
As observed in follicle cells, expression of rab9CA significantly suppressed the NMJ morphology defect in lrrk NS flies (Fig. 7J vs 7H; Fig. 7K).
The vulval morphology defect in hda-1 animals suggests that hda-1 is involved in cell differentiation and cell migration processes.
DOI: http://dx.doi.org/10.7554/eLife.07739.019 To further test this idea, we examined the relationship between respiratory complexes and the mitochondrial morphology defect in ∆MICOS cells.
Enhancing rab9 activity also rescues the NMJ morphology defect in lrrk NS flies, which have an NMJ phenotype identical to that reported with loss-of-function of the retromer component Vps35 (Korolchuk et al., 2007).
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