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Linear growth and vegetative morphology assays revealed no significant differences between sad-3Δ and wild-type strains.
The genetically engineered phage can be used as novel phage-based array chips that are optically readable for cell proliferation and morphology assays.
The ability to automate morphology assays on 3D cell cultures is a powerful tool, but some scientists are now pushing the technology even further, with algorithms that can classify structures in living animals and clinical specimens.
Cells were then incubated for 48 hours prior to β-catenin localization, viability, PCR, and morphology assays.
To evaluate whether MB pretreatment has a protective effect on hair cell death after noise exposure, we performed two types of hair cell morphology assays.
Proliferation assays were done by cell counting, cell cycle assays by propidium iodide staining, and morphology assays by image analysis of tetramethylrhodamine isothiocyanate-phalloidin-stained cells.
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These alleles neither appear to mitigate general apoptosis (assayed with GMR- hid) nor have any intrinsic effects on eye morphology (assayed with GMR-GAL4 alone).
In the morphology assay, we found that DCX knockdown also induced excessive axonal branches compared with control neurons (Fig. 4G and 4H).
Surface morphology assay demonstrated that HTCC/GP hydrogels were well constructed with three-dimensional (3D) porous structures in the range of 5 of 40 μm.
Flow cytometry demonstrated the accumulation of subdiploid cells (Fig. 2B), and the nuclear morphology assay showed nuclear condensation (Fig. 2C).
Cell morphology assay revealed a strong likelihood of "massive" apoptosis (cell shrinkage, and fragmentation into membrane bound apoptotic bodies) in the CSE treated cells (Fig. 4A, F inset) as opposed to βGA/MFA/control groups.
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