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Although villus morphology appeared normal at E16.5, the first time at which both Gata4 and Gata6 were efficiently reduced, changes in expression of markers of enterocytes, goblet cells, and proliferative cells were detected.
Under these conditions, the first polar body extrusion was restored up to 72% and both meiosis I and II spindle morphology appeared normal.
Bone morphology appeared normal, as was a foetal echocardiogram.
In sec8P1 and sec8Δ1 mutants, NMJ morphology appeared normal in first instar (L1) larvae (Fig. 3A).
In both environments, morphology appeared normal, with the exception of stage 14 when the anthers typically extend above the stigma so that pollination can occur (Fig. 5).
Characterisation of meiotic divisions showed that spindle poles and spindle morphology appeared normal and we never detected chromosome segregation defects (data not shown).
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However, it had previously been shown that CaP motoneuron axon morphology appears normal when muscle pioneers are ablated in zebrafish embryos (Melancon et al., 1997).
However, circulating reproductive hormone levels and ovary morphology appear normal in adult mice and Trpc2-/ females, with degeneration in both VNO layers, are not reported to have irregular estrus cycles [ 5].
IHC stereocilia morphology also appeared normal throughout the cochlea in the mutant (Supplementary Information Figure S1b) consistent with the absence of gelsolin in IHCs.
Immunohistochemical analysis of TH::htauWT brains at different ages revealed that the morphology of DA neurons appeared normal in the first week, with no detectable morphological changes in the soma and neurites, suggesting that the neuronal architecture was normal in young adults (Fig. S4).
Moreover, the diaphragm did not undergo degeneration/regeneration cycles and its morphology appeared near normal in 5-week-old and 4-month-old animals (Fig. 6A C).
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