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Histological sections stained with hematoxylin & eosin, and Alcian blue confirmed chondrocyte morphology and matrix formation.
These analyses clearly show a positive correlation between opaque colony morphology and matrix formation.
The cell morphology and matrix staining indicated good repair with 4 injections in the intermediate and high-dose subgroups.
To assess cell morphology and matrix deposition, alginate beads were fixed in 3.7% formalin (Merck) + 100 mM CaCl2 (Calbiochem, Darmstadt, Germany).
In the present study, we investigated the effects of tumor necrosis factor alpha (TNF-α) and epidermal growth factor (EGF) on chondrocyte morphology and matrix gene expression.
Examination of long bones showed changes in the morphology and matrix composition of the growth plate, most notably in the thickness of the hypertrophic chondrocyte zone, which was reduced by up to 50% [ 35].
Similar(54)
In this work, we probe the viability and adhesion of osteoblasts on ordered mesoporous materials with different morphologies and matrix chemistries.
Bovine chondrocytes were used to assess the biocompatibility of the scaffolds over 21 days under two-dimensional (2-D) and three-dimensional (3-D) cell culture conditions, particularly looking at cell morphology, proliferation and matrix deposition.
We hypothesized that plaque levels of Cav-1 might be related to plaque morphology, inflammation and matrix metalloprotease (MMP) activity.
This is attributable to the difficulty in generating 3-D matrices with controlled morphology, rheology, and matrix composition, and a lack of established methods to visualize and evaluate cell functions over prolonged periods.
The effects of serum-free media, growth factors and culture system on cell proliferation and morphology and extracellular matrix (ECM) synthesis were evaluated.
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