On nonlinear strain-stiffening fibrin gels, the same cell types become maximally spread even when the low strain elastic modulus would predict a round morphology, and cells are influenced by the presence of neighbors hundreds of microns away.
In contrast, rapamycin did not cause senescent morphology and cells resumed proliferation, when rapamycin was removed.
The presence of CaCl2/CFW in the growth medium influenced cellular morphology and cells grew as pseudohyphae (Fig. 4I).
Cell labeling would have shed a light on the final cell ratio, possible changes in cell morphology and cells secreting proteoglycans.
After 10 days of neuronal induction, differentiated cells exhibiting neural-like morphology and cells positive for nestin expression, which is generally used as a specific marker for early neural progenitors, could be observed in the hUCMSC cultures).
In MEL-10 and Mel-9, two melanoma-derived cell lines, nutlin-3a induced p53 and p21 and caused senescent morphology and cells did not resume proliferation, when nutlin-3a was removed (Supplemental Figure 2).
