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We showed that these proteins are crucial to define the type of morphology a phage likely adopts.
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Based on this morphology, a number of other phages from the literature can be classified as probable viunalikeviruses (Table 1; Supplementary Figure 1).
In a extensive review of morphology of phage particles carried out by Ackermann [32] the closest-related Xanthomonas genus have at least 35 types of tailed phages, where 25 (72%), 9 (25%) and 1 (3%) and are from Siphoviridae, Myoviridae and Podoviridae families respectively.
Thus, this clearly shows that classification of phages solely based on morphology is not sufficient to determine to which family a phage should be assigned, but that genomic data is also necessary.
Villarroel, J. et al. HostPhinder: a phage host prediction tool.
Phage display is based on expressing recombinant proteins or peptides fused to a phage coat protein.
a Phage display technology.
Phages prepared with this construct were analyzed as was described for M13-Cry1Ac. Figure 3a shows a phage blot assay showing that Cyt1Aa protoxin is displayed on filamentous phages.
Fig. 2 Cartoons of a the anatomy of a phage and b the life cycle of a phage.
Phage peptide display is a selection technique in which random peptides from a library are expressed as a fusion with a phage coat protein, resulting in a display of the fused protein on the surface of the phage particle.
Fig. 3 Results of a time-shift experiment showing rates of a phage infectivity and b bacterial resistance evolution in coevolving populations of P. fluorescens and phage.
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