Similar cell morphologies were labeled in the absence of exogenous T3.
For tooth-diet and toothrow-diet sets, morphologies were labeled according to the dietary preferences of the corresponding species (estimated from the literature [25]): vertebrates (V), invertebrates (I), grass/foliage (G F), and seeds/fruits/succulent plant tissue (S-F-SPT).
In agreement with previously published literature, both neurons and glial cells were loaded with OGB-1 and cells with astrocytic morphology were labeled with SR101 (Figure 1A) [21], [21].
Vulval muscle morphology is labeled by mCD8::mCherry.
Of 96 colonies tested, 9 with good morphology passed both tests, and were labeled C1-C9.
However, the few cells that were labeled in both low and high T3 concentrations shared a progenitor-like morphology (Fig. S4E-F') unlike the cells labeled by control vector (Fig. S3E).
In both types of cases, abundant neurons were labeled throughout the rostrocaudal extent of the ipsilateral TLCd and they showed similar morphology and distribution (Fig. 11c d).
The nuclear marker DAPI (4′,6-diamidino-2-phenylindole) was used to counterstain DNA and cells defined as apoptotic if the nuclei showed a sharply demarcated morphology and were labelled by the TUNEL assay, were increased 3.3-fold in diabetic rats, although the overall incidence of this abnormality was very low [ 160].
Moreover, geometrical graphs, which are labeled with the spatial relations of the graph nodes (Pach, 1999), can be employed in order to formalize the geometrical properties of morphologies.
Each neuron in C. elegans' nervous system has distinct properties in view of morphology, connectivity, and position, and therefore it can be labelled specifically.
These morphologies were selected based on labeling method (intracellular dye injection) and reconstruction quality (400-µm sections were used and distal dendrites included in reconstruction) and files downloaded from Neuromorpho.org (cell IDs M. fascicularis: cnic_019 024 and cnic_055 060, M. mulatta: cnic_007 012 and cncic_050 054 (Duan et al. 2003)).
