During this same period, five specimens of intermediate morphology were collected, suggesting possible hybridization.
Three-dimensional data on basicranial morphology were collected by one of us (DHD).
Because MACS/matrix enrichment generates a heterogeneous population of cells dominated by spermatogonia, cells with typical spermatogonial morphology were collected with a micromanipulator for microarray and real-time PCR analyses to measure gene expression profiles of adult human spermatogonia during short- and long-term culture.
Additionally, soot particles were thermophoretically sampled from the flame and soot morphology data were collected with a transmission electron microscope (TEM).
To assess postnatal renal morphology, kidneys were collected from wildtype and betaglycan+/− PN4, PN5, PN6 and PN30 mice, immersion-fixed in 10% neutral buffered formalin, processed and embedded in paraffin wax.
Parasite morphology data were collected by re-examination of the original blood films for a small subset of samples; travel histories, demographic data and records of chemoprophylaxis use were sourced from the MRL malaria surveillance database.
Historical data on land use, channel morphology and sedimentology were collected, based on a spatial analysis of aerial photographs (1963 1995) and a field survey (2002).
To demonstrate altered islet morphology, pancreas sections were collected and stained with hemotoxylin and eosin.
The remaining 7%, 12 species, were identified solely from video observations of their morphology, since no specimens were collected.
Clinical information on the exposure to known or suspected risk factors such as age, gender, anatomical site, lesion morphology, and smoking/alcohol history were collected.
