In comparing this morphology to spontaneous differentiation morphology, BMP-treated cells were usually bigger and homogeneously shaped, while spontaneous differentiation tended to be restricted to discrete spots, not synchronic and morphologically heterogeneous (data not shown).
After multiple freeze-thaw cycles (≥ 20), fluorescent protein [ 24] was stably expressed in A375-G6PD∆ A375-G6PD∆∆-G6PD-WT, A375-G6PD∆-G6PD-WTD-G487A cells, which were morphologically normal (data not shown).
Notably, skeletal muscle, including diaphragm, was also morphologically normal (data not shown), suggesting that the cause of death may be due to a functional impairment of muscles required for breathing and suckling.
One mutant, Δ ppm1, formed morphologically normal gametocytes (data not shown) and produced macrogametes that emerged from their host cells expressing the activation marker P28, but did not produce any ookinetes.
In addition to one case, which was detected by Southern blot analysis, two patients were found to produce a poor amplification with the M primers in morphologically normal tissues (data not shown).
Srpk, pk7, and gak mutants formed morphologically normal gametocytes (data not shown), and ookinete cultures contained macrogametes that had emerged from their host cells expressing the activation marker P28 (i.e., Figure 4A), suggesting that neither gametocytogenesis nor early events in macrogametocyte activation were responsible for the lack of ookinete production.
Prolongated culture on PCZ supplemented media led in many cases to the disappearance of a morphologically visible shoot meristem (data not shown).
Injection of mpv17 and mpv17 EGFP encoding mRNAs into tra mutant embryos lead to the development of iridophores in the retina which are numerically and morphologically indistinguishable from wild type (data not shown).
However, these pathological changes had almost completely improved 168 h after reperfusion, as the H&E-stained liver tissues were no longer morphologically distinguishable between the two groups (data not shown).
Imaging analysis by electron microscopy showed that the pVII modified virions were morphologically identical to the unmodified virions (data not shown), ruling out that the pVII modification introduces any gross physical alterations of the virions as compared to their unmodified counterparts.
