In the experiments described herein, the nonadherent cell population consisted of greater than 90% morphologically apoptotic cells following treatment with all the NSAIDs except rofecoxib.
This methodology revealed the presence of 47 CTCs (EpCAM +, CK +, and CD45 -) in the 7.5 ml blood sample, some with morphologically apoptotic features, and over 13,000 CTCs (EpCAM +, CK +, and CD45 -) in the 5 ml ascitic sample.
Increased p53 and APLP1 staining colocalized with cells showing morphologically apoptotic features, suggesting the possibility that Mn may function through p53 to affect changes in APLP1 that promote extracellular A β accumulation.
However, there were almost no TUNEL-positive or morphologically apoptotic cells in U87-MG cells treated with anti-PDGF or control antibody.
Compared with control cells, MRP-AS transfectant cells demonstrated a higher proportion of dead and morphologically apoptotic cells, spontaneous neuritogenesis, and, increased synaptophysin and neurofilament expression.
The percentage number of morphologically apoptotic cells was counted by fluorescence microscopy as described (Ko et al, 2002).
Fifty-six dafterfter instillation, morphologically apoptotic cells could be identified in granulomatous cells of lung tissue from silica-instilled rats.
The most differentiated tumour cells, which often express p53, and occasionally p21 and Bax, lie adjacent to TUNEL-positive, morphologically apoptotic cells.
In contrast, 5-FU did not cause internucleosomal DNA cleavage by 48 h at any concentration, despite the presence of morphologically apoptotic cells 24 h earlier.
Additionally, to compare results with cutaneous lesions of SLE patients, 20 biopsies of lupus erythematosus (LE) skin lesions were analysed morphologically for apoptotic cells and infiltrate.
