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sigmar S mutant salivary glands are morphologically abnormal with large empty vacuole-like structures, tubulin disorganization, and reduced autophagy flux.
Here we demonstrate that in sigmar loss-of-function mutants, the salivary glands are morphologically abnormal with defects in the tubulin network and decreased autophagic flux.
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The majority of giant cells were surrounded by morphologically abnormal astrocytes with long processes typical of interlaminar astrocytes.
To assess the utility of the pipeline we used zebrafish mutants carrying loss of function alleles from the Zebrafish Mutation Project (ZMP) [ 16] and compared morphologically abnormal embryos with normal sibling embryos.
Electron microscopy demonstrated morphologically abnormal mitochondrial cristae with enlarged cisternae, which were predominant in the Pank2 −/− (Fig. 6F H) when compared with Pank2 +/+ (Fig. 6E G).
It was found that in treated TCBZ-S flukes, strong positive labelling indicating apoptosis was associated with morphologically abnormal cells undergoing mitosis or meiosis in the testis, ovary and vitelline follicles.
However, under control conditions, we also identified 12 metaphases (24%) with morphologically abnormal alignment.
First, there was an association with marrow involvement (P less than 0.05) which usually accompanies involvement of the blood with morphologically abnormal cells.
The diagnosis of endometrial hyperplasia is based on microscopic findings of a morphologically abnormal proliferative-type endometrium, with some authors insisting that there must also be an abnormal increase in endometrial volume[ 22 ].
The kidneys from such embryos showed effects ranging from those with severely reduced and morphologically abnormal branching and growth to those with more normal ureteric bud trees.
In the pupal stages examined, all of the mutant salivary glands were significantly longer and wider (supplementary material Fig. S4C,D) compared to wild-type glands and were morphologically abnormal (compare Fig. 3A,B) with some of the cells appearing grossly enlarged with big empty vacuole-like structures (Fig. 3B).
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