When the selective media were insufficient for the determination of the pathogens' species, the isolates were subsequently subjected to micro-morphological and physiological tests using the fungal identification kit API20 (bioMérieux Vitek, Inc., Hazelwood, MO, USA).
However, there is currently no implementation available for these tests using morphological data in a parsimony framework.
In practice sexual and apomictic plants are differentiated by i) cytological analyses of ovule development [ 23], ii) progeny tests using morphological or molecular markers [ 24], and iii) flow cytometry of seed nuclei to identify distinguishing embryo to endosperm ploidy level ratios [ 25].
Different combinations of feature groups with morphological features were tested using the three classifiers and their average performance was also computed.
The proposed MCS combines the results of two classifiers trained and tested using dynamic and morphological features separately.
Colonies of bacteria with morphological characteristics compatible with C. pseudotuberculosis were tested using API Coryne Strip (API CS; bioMérieux, France) according to the manufacturer's recommendations.
To investigate the associations between the genetic and morphological characterisations, a contingency analysis was performed with the likelihood ratio chi-squared test, using the JMP 7 software.
Although the one sample test using the natural strains identified 36 heteroclite mutants out of 110 mutants, the average Mahalanobis distance from the parental strains was not significantly different (Mann–Whitney U-test, P <0.05; Figure 4B), suggesting that the difference between these groups was not due to the extent of morphological difference but rather to different morphological profiles.
For the analysis of morphological parameters at the microcalcification level (414 microcalcifications associated with malignant lesions and 183 associated with benign lesions), the normality of the data was tested using the one-sample Kolmogorov-Smirnov test.
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