In contact-inhibition, beta-Gal staining, a marker of lysosomal overactivation, is mTOR-independent [ 41] and Figure 1 C. We next, employed two non-morphological tests to evaluate senescence: (a) cellular hypertrophy, measured as protein per cell and (b) the reversibility potential (RP) or the potential to restart proliferation and to regenerate cell culture after splitting in drug-free media.
However, relatively few biochemical and morphological tests are available to distinguish L. sphaericus as a different species.
Atomic force microscopy (AFM) was employed for the morphological testing.
Morphological testing of these 12 isolates was performed with HiMedia bacterial kit for the biochemical tests.
Through our morphological testing, we determined that two of the 24 viable mutants had no obvious growth defects (Δ pty-6 and Δ pph-7).
All cell lines were subconfluently grown and passaged, routinely tested for mycoplasma contamination and subjected to frequent morphological tests and growth curve analysis as quality-control assessments.
After oxidation, the coatings were investigated by a series of structural, morphological, and tribological tests to understand the effect of CNTs addition to the base electrolyte.
Poore (2005: 2) [ 3] stated that his morphological data set was "essentially a compilation of those [morphological characters] used previously but with additions", which leads to the reasonable expectation that Poore's analysis should be the most severe morphological test of peracarid (and possibly wider eumalacostracan) phylogeny published to date.
Morphological tests, chemical techniques, and scattering techniques were used to study the interaction mechanism between graphene and Nafion, resulting in great improvements of the actuation performances.
However, to date, the question of the better way to evaluate tumoral PARP-1 activity (functional cytosolic assay as in our study, or morphological test such as IHC) is still open [ 13, 14, 41].
