This study confirms and extends prior morphological studies that have reported age-related dimensional changes in the corpus callosum and, in addition, provides evidence of age-related biophysical tissue differences in the human corpus callosum.
In contrast to morphological studies that evaluate developmental constraints qualitatively by morphological resemblance among embryos, our genome-based method evaluates developmental constraints quantitatively in a single species.
The molecular data have been confirmed by morphological studies that found differences in wing patterns among at least 3 taxa [ 61].
The observed similarities confirm results of prior morphological studies that reported the antennae of both species share similar sensilla densities overall [ 62].
However, there are other postmortem morphological studies that have reported evidence that strongly supports the theory that the peripapillary choroid does not supply the prelaminar region of the optic nerve head [ 23- 26].
One reason that the archezoa hypothesis was good is that it too raised a number of testable predictions, and the phylogenetic and morphological studies that addressed them moved the field along positively.
Indeed, this conservation is in keeping with previous, comparative morphological studies that reported a slightly higher density of sensilla on An. gambiae antenna, including the highly abundant trichoid sensilla [ 62] that house three Orco-expressing ORNs [ 13, 16].
Our isolation of the ERGIC as the locus of LC3 lipidation is in line with morphological studies that describe an omegasome structure projecting directly from the ER membrane (Axe et al., 2008; Hayashi-Nishino et al., 2009).
A clade containing Hemerocoetinae and Creediidae, as resolved in the molecular phylogeny, was also hypothesized from phylogenetic analysis of 61 morphological characters [ 60], and is a result that was predicted in other morphological studies that did not rely on optimization of discretely coded character states [ 33, 61- 63].
Within the last ten years, resolution and suitability of modern imaging techniques, among them confocal laser scanning microscopy (CLSM), synchrotron radiation micro-computed tomography (SRμCT), micro-computed tomography (μCT), and magnetic resonance imaging (MRI), have led to a considerable increase in morphological studies that generate entirely digital raw data [e.g. [ 7- 10]].
