For more than a century, the majority of morphological studies in neuroscience at the cellular and subcellular level have been carried out using the Golgi approach to stain individual neurons [1] and visualize their dendrites and dendritic protrusions.
The biopsy tissue was divided and either processed for morphological studies, or stored at -80°C for future molecular study (e.g. gene expression studies).
Images suitable for morphological studies were obtained at Ex 860 and 890 nm and included SHG-B and SHG-F signals (Additional file 11: Figure S9A).
Morphological studies of SPNs at the electron microscopic level have revealed that the majority of dopaminergic synapses terminate on the neck of spines that receive excitatory inputs from cortex and thalamus (Wickens and Arbuthnott, 2005).
Morphological studies have revealed that at least two types of parvalbumin-expressing interneurons in the hippocampus: large, multiple-polar round shaped, axon-somal basket cells and smaller, spindle shaped, axo-axonic chandelier cells [28].
An ongoing morphological study looks at non-truncated characters of Paedocypris and compares them with other cyprinid representatives (Britz and Conway in prep .. Our phylogenetic analyses of a large number of cyprinids consistently recovered Sundadanio as the sister group of Paedocypris.
For morphological studies, the brains were perfused at the end of the experiment via the basilary artery with a solution of paraformaldehyde-lysine-periodate (PLP) in 0.1 mol/l phosphate buffer (PB), ph 7.4 for 10 min. Post-fixation was carried out in PLP for 2 h at RT.
Moreover, because of the lack of detailed morphological studies in many groups, especially at the microscopic level, even in extant forms it may be difficult to determine whether or not some characters have evolved relatively independently (e.g., U-shaped gut or tentacles).
Therefore, all morphological studies on variations of structures at the suprascapular region are potentially helpful in the diagnosis and treatment of this pathology.
Several morphological studies report appressorium-like swellings at the tip of invading Z. tritici hyphae (Cohen and Eyal, 1993; Duncan and Howard, 2000; Kema et al., 1996a; Fig. 3).
