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For the ease of description and comparative evaluations from different published results, morphological stages of a protocorm or a plantlet in one's study should be clearly defined.
Total RNAs (including small RNAs) were extracted as described before, from the 4 individual morphological stages of young panicles of O. glaberrima (CG14) and O. barthii (B88).
To test these hypotheses, expression analysis of miR2118, 21-nt phasiRNAs and their RNA precursors was performed on 4 distinct morphological stages of panicles collected from O. barthii (accession B88) and O. glaberrima (variety CG14) (Fig. 3; see Additional file 10 for histological description).
Our spatial expression analysis of miR2118, phasiPH12-1 and PH12 lncRNA and their differential accumulation patterns with respect to the morphological stages of panicle development between the two species indicates that at the branching stage, the gamete-associated small RNAs are initiated later and expressed at lower levels in O. glaberrima than in O. barthii.
The different morphological stages of the differentiating cells were documented with an ocular camera (Microscope Systems).
The 48 hour Pf IDC consists of the morphological stages of ring, trophozoite, schizont and merozoite.
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This suggests that for the same morphological stage of panicle development, stamen differentiation, and therefore floret differentiation, might be delayed in O. glaberrima with respect to its wild relative, O. barthii.
While neither mature miR2118 nor 21-nt phasiRNA phasiPH12-1 were detected at the early morphological stage of panicle development (inflorescence meristem after reproductive transition, stage 1), their expression was initiated earlier in O. barthii (early branching stage, stage 2) than in O. glaberrima (late branching stage, stage 3).
ACF represent the earliest morphological stage of colorectal tumorigenesis (McLellan et al, 1991).
Animals were age-matched for all experiments using timed laying plates and morphological staging of late-stage embryos.
However, early postpartum weight might be less important for the prediction of maternal obesity as weight at 6 weeks postpartum may be affected by edema, morphological stage of reproductive organs and lactation compared to 6-12 months [ 46].
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