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A clear spatial separation of actin and flotillins occurred as early as 5 minutes after stimulation (Fig. 1d), before any morphological separation of leading and trailing edges became apparent.
Additionally, the explicit morphological separation of traditional Basmati and non-Basmati aromatic landraces in the PCA scatterplot is another significant outcome of our analysis.
This feature is consistent with the morphological separation of the two subclades within Koerneria, as already discussed by Fürst von Lieven [ 23].
Although the Leptsomatidae and Anticomidae are both placed within the Enoploidea, these taxa were never recovered as a monophyletic clade, supporting the morphological separation of these two groups.
Morphological separation of species is often extremely difficult without previously determined specimens on hand, requiring subjective interpretation of variable characters such as cuticle punctation or coloration [ 40].
Floyd [ 28] pointed out the difficulty with the morphological separation of Oecetis larvae and the Churchill DNA results support that conclusion.
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Because of the doubtful morphological separation from its supposed closest relative, Cucumella [ 12, 13, 18], and the insufficient sampling of other potentially related genera, the status of Cucumis as a monophyletic genus has remained equivocal.
Intriguingly, this pattern of variation was mirrored by the more pronounced morphological separation between the diploid and tetraploid taxa than that seen between species at either ploidy level.
In particular, morphological separations or affinities were confirmed through comparisons between the species-groups.
Schefter and Wiggins [ 23] provided morphological characters for separation of the three known Churchill Hydropsyche species, which should be examined if additional color variations are found.
Proliferative lesions of the preputial glands comprise a morphological continuum and separation of these growths into categories of hyperplasia, adenoma, and carcinomas is based largely on cytological features and the degree of altered growth patterns.
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