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The morphological separation between the gossypii group and the other species-groups are congruent with their phylogenetic relationships.
Intriguingly, this pattern of variation was mirrored by the more pronounced morphological separation between the diploid and tetraploid taxa than that seen between species at either ploidy level.
The clear morphological separation between the two species argues against continued events of gene flow between them, which if present should have produced a higher frequency of specimens with intermediate morphologies.
Both the fossil record and reconstructions of the ancestral morphotype support the idea of a more generalized ancestor, with a progressive morphological separation between two related clades, the 'slow-climbing' lorises and the 'fast-leaping' galagos [ 46, 47].
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A clear spatial separation of actin and flotillins occurred as early as 5 minutes after stimulation (Fig. 1d), before any morphological separation of leading and trailing edges became apparent.
This feature is consistent with the morphological separation of the two subclades within Koerneria, as already discussed by Fürst von Lieven [ 23].
In particular, morphological separations or affinities were confirmed through comparisons between the species-groups.
Within the Tripyloididae, morphological evidence seems to support the observed separation between Bathylaimus and Tripyloides in 28S phylogenies (Additional file 2, Figure S2).
This result, based on morphological characters, agrees well with the reciprocally monophyletic separation between the gossypii group and the cluster of the other three groups in the molecular phylogeny (Figure 1; [15], [15]).
However, although our analysis showed a clear separation between Neanderthals and early/recent modern humans and morphological proximity between European specimens from OIS 7 to 3, it also shows that the European hominins from the first half of the middle Pleistocene still shared most of their cranio-facial architecture with their African contemporaries.
The dendrogram from the global analysis (50 morphological features, Figure 2A, for more details see Figure S3A) displays a clear separation between the cluster which includes al modern humans and the other specimens based on chi-square dissimilarity index and which indicates a high degree of dissimilarity (dissimilarity value = 0.334).
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