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Taken together, these data suggest that the primordial germ cell, epiblast, and hypoblast precursors were already molecularly specified from early-blastula stages, prior to morphological segregation of these cell types.
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Similar to mouse pre-implantation embryos (around E4), prior to epiblast epithelialisation, zebra finch embryos at oviposition are at early mid-blastula stagearly mid-blastulahow no overt morphological stagesation despite the expression of epiblashowno primitive endovert/hypoblast morphologicalsegregationpopulations of cells with the embryo.
The high level of heterozygosity in artichoke has been demonstrated by Basnizki and Zohary (1994) who reported that selfing clonally propagated artichoke varieties leads to a high level of morphological segregation in the offspring, accompanied by considerable inbreeding depression.
Our study suggests that the single case of altitudinal morphological segregation within a species in the Eastern Arc Mountains is likely the result of secondary contact of two highly differentiated lineages instead of disruptive selection across an altitudinal gradient.
Consequently, 18 plants homozygous for the double-genes, Xa7 plus Pi2, were obtained in MF3 and they exhibited little morphological segregation and high similarity to Guangzhan63-4S.
At EGK-X, clear epithelial organization can be visualized, with polarized E-cadherin and morphological segregation in the bright-field image (F ).
Gene expression at early- and mid-blastula stages was not associated with any overt morphological segregation at either EGK-VI or EGK-VIII.
(iii) If significant genetic diversity is present, is there morphological, geographical and/or ecological segregation of the different major lineages?
At least two factors may result in segregation of morphological variation into groups that do not correspond to geographic variation or genetic divergence.
We found similar patterns for mouse gene expression levels, Arabidopsis life-history traits, and yeast morphological phenotypes, implying that this segregation of buffering effects is not limited to any one particular class of traits or species.
Moreover, collecting seeds and raising seedlings from the putative first generation hybrids would allow investigating the segregation of the morphological traits involved in species differentiation.
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