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Gross morphological screening was based on classical forward genetic criteria [ 8, 9] and initially focused on morphological landmarks readily visible via a dissecting microscope.
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As spontaneous DH frequency was low, later screens emphasized H recovery where the morphological screen was followed by flow cytometry; homozygosity of candidate Hs was thereafter confirmed with microsatellites.
The resulting morphological screening pipeline was conducted on multiple days during the weekly screening workflow, with most screening conducted from 1 through 3 days post fertilization (dpf) based on the high efficacy of MOs during this developmental time-period [ 4, 6].
The screening was done by morphological observations throughout the development of the transformed plants for phenotypes such as fruit length and shape.
Antibody screening was negative.
The screening was electric.
Morphological screening in zebrafish was pioneered through chemical mutagenesis genetic screens [ 8, 9].
Screening is virtually nonexistent.
Screening is relatively inexpensive.
Although the screen used by Seiler and Plamann identified morphological mutations in NCU00651 with high frequency, their screen was not designed to find TS lethal mutations.
The screen was clean.
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