Morphological screening in zebrafish was pioneered through chemical mutagenesis genetic screens [ 8, 9].
The resulting morphological screening pipeline was conducted on multiple days during the weekly screening workflow, with most screening conducted from 1 through 3 days post fertilization (dpf) based on the high efficacy of MOs during this developmental time-period [ 4, 6].
However, some stamens from transgenic plants can still produce pollen and morphological screening, in vitro germination and in-situ pollen germination were performed to determine the activity of this pollen.
Gross morphological screening was based on classical forward genetic criteria [ 8, 9] and initially focused on morphological landmarks readily visible via a dissecting microscope.
The results from the morphological screen underscore the importance of including these basic, easily observed criteria in the MO screen to identify novel regulatory functions of sensory organs and pigment cell development.
As spontaneous DH frequency was low, later screens emphasized H recovery where the morphological screen was followed by flow cytometry; homozygosity of candidate Hs was thereafter confirmed with microsatellites.
We used the results of a genome-wide RNAi morphological screen in Drosophila melanogaster S2R+ cells [ 8] to select genes that altered the shape of S2R+ cells, but for which the function in Drosophila was not previously known.
For the purpose of initial staging at diagnosis, morphological investigations by cytological screening of bilateral BM aspirates and histological assessment of bilateral core biopsies are recommended.
In conclusion, we have developed an automated quantitative analysis of neuronal development that allows a more rapid screening of morphological characteristics with a high degree of accuracy and reproducibility.
At the physiological level, we confirmed through extensive, although laborious, in-field screening of various morphological properties to ensure that DXT and DXB were morphologically equivalent or similar.
