Complete information on the number of cells and morphological score on days two and three, ICM score, trophectoderm score and expansion status was available for 206 embryos.
We validated morphological scoring of apoptosis against the terminal deoxynucleotide transferase labelling (TUNEL) technique (ApopTag; Oncor, Gaithersburg, MD, USA) (Bach, et al 2000).
The pancreatic islets were evaluated blindly with semi-quantitative morphological scoring of fluorescent intensity: 0, no or very weak Ogg1 staining; 1, moderate Ogg1 staining; and 2, strong Ogg1 staining.
Accordingly, we evaluated the immune-morphological score of each embryo generated from each patient's cohort of oocytes collected after ovarian stimulation.
The morphological scores on Day 5 showed a similar trend.
Histo-morphological scoring of adjacent H&E-stained sections was used to assess the severity of disease.
For the morphological score, the degree of fragmentation of the embryo and the uniformity of the blastomeres were assessed daily.
There was no significant association between male age and the average morphological score, with a mean decrease of 0.02 [95% CI 0.002 to −0.044] (P = 0.07).
Logistic regression analysis was performed to assess the association between number of cells on day two and three, morphological score day two and three, ICM score, trophectoderm score and expansion status with the success of establishing a new hESC line.
The number of independent components (ICs) (chemical constituents) in each peak cluster was estimated using morphological score method.
