In nucleated patch recordings, the success of morphological reconstruction from biocytin-filled cells was relatively low, thereby hampering the identification of cell morphology and NG2 immunoreactivity.
Shortly thereafter, Binzegger et al. (2004) collected layer-specific data on the distribution of boutons and dendrites of in total 13 cell types from morphological reconstructions of in vivo-labeled cells from area 17 of the cat.
Morphological reconstructions were produced manually from multiple image stacks.
This was achieved using morphological reconstructions of h-dome images from seeds corresponding to pixels of intensity h.
The estimated lateral spread is consistent with data from rat and cat primary visual cortex, which were obtained based on morphological reconstructions and the potential connectivity method: Hellwig (2000, his Fig. 7), reports a lateral spread of 150 310 μm, and Stepanyants et al. (2008, their Fig. 7), find a spread of around 200 μm of main projections in input and output maps.
We compared LFPs generated by the morphological reconstructions of these neuron types described by Mainen and Sejnowski (1996)—hereafter referred to as Mainen cells with the LFPs from reduced versions of these models created using Bush and Sejnowski's method (1993)—hereafter referred to as Bush cells.
We examined this possibility by modeling the response to WD in 6 different L2/3 PCs for which full morphological reconstructions were obtained in vitro.
The CaP motor neuron was also co-loaded with 40 μM Alexa Fluor 647 hydrazide (Invitrogen, Eugene, OR) to identify the boutons and perform morphological reconstructions.
Range III: cycling with lower-potential end below 0.0 V results in obvious capacitance reduction, along with different morphological reconstruction and Mn reduction from those in Range II.
DOI: http://dx.doi.org/10.7554/eLife.01206.010 To determine whether the delay in calcium signal onset correlated with the physical distance of the boutons from the proximal axon or soma, we combined calcium imaging with morphological reconstruction of the motor neuron.
