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Based on a comparative morphological procedure, we found that this leaf pattern was formed by the NGP common elements.
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Using both biochemical and morphological procedures, we confirmed the presence of integral components of lipid droplets (both lipids themselves and structural proteins associated with the lipid droplets) in autophagosomes and lysosomes.
Thus, the validity and reliability of our novel morphological procedure have been confirmed successfully by the use of an independent, conventional ELISA method.
The morphological procedure is therefore used on these binary images to refine the margin and content of the tumor images.
Identification of the NGP elements was conducted based on Remane's criteria, a validated comparative morphological procedure used in systematic studies [ 45].
Although the NGP of the wing patterns was rigorously identified by a comparative morphological procedure, such determinations of homology remain hypothetical because of the lack of developmental genetic data.
Thank to this procedure we gained as much morphological information as possible from the tissue specimen that was homogenized for molecular analysis.
Despite the morphological procedures applied here in cleaning up the output images (see Materials and Methods section below), the resulting maps may still contain a few artefacts.
In addition, these morphological procedures are often sensitive to the orientation of a worm body, as exemplified by Figure 4c.
Taking into consideration that the changes of these parameters are interrelated under specific physiological and pathological conditions, an overall quantification of their values would enhance our understanding of the hemodynamic and morphological procedures under changes in metabolic demand.
To ascertain the degree of global morphological variance, we performed principal components analysis (PCA), a statistical procedure that uses an orthogonal transformation.
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