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To determine when these morphological phenotypes occurred, we tracked individual 4 dpf Tg(fabp10 dsRed) larvae exposed to 350 mM ethanol over 32 hours (Fig. 1C) and verified these findings in larger cohorts (Fig. 1D,E).
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Once transformed to the PA6-X phenotype, reversion to the PA6-DA morphological phenotype did not occur.
While EMT is structured, following extensive alterations in gene transcription, transition between the mesenchymal and amoeboid phenotype occurs rapidly, involving morphological transition, with concomitant changes in migratory behaviour arising in response to micro-environmental changes.
More importantly, zebrafish offers the advantage of high-throughput scale in the study of RUNX1-Evi-1 function and drug screen in vivo, which enables us to track the molecular alterations that occur well before the appearance of morphological phenotypes, and to determine the roles of candidate RUNX1-Evi-1 target genes.
However, we also detected partially reprogrammed colonies with non-hES morphological phenotypes, where transgene silencing, based on GFP expression, did not occur (Figure 1A, third column).
C1-C3 Manually identified 3D cell centers and morphological phenotypes corresponding to B1-B3.
Oncogenes introduced into MCF-10A cells disrupt this morphogenetic process, and elicit distinct morphological phenotypes.
This inducible heterophylly of M. aquaticum leads not only to two different morphological phenotypes (see Figure 1B,C).
The morphological phenotypes included shortened flagella, increased incidence of spheromastigotes, agglutination and a novel walnut-like appearance.
It is suggested that sporulation relay may have impact on morphological phenotypes as well as metabolism.
A similar design was employed to assess the temperature dependence of observed morphological phenotypes.
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