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The morphological phenotypes due to Vps35 depletion in developing CA1 neurons suggest a dependence of VPS35/retromer in hippocampal neuron morphogenesis in vivo.
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To further validate that these morphological phenotypes are due to disruption of esco2, we have recently generated an esco2 exon 3 frameshift mutation (esco2+13), using the CRISPR genome-editing system (Hwang et al., 2013; Jao et al., 2013; Thomas et al., 2014).
However, not all mutants defective in putative metal transporters display morphological or ionomic phenotypes due to the complexity and redundancy of the elaborate metal transport system.
That morphological phenotype might be due to FSHD myoblasts being more sensitive to stress than analogous controls.
C1-C3 Manually identified 3D cell centers and morphological phenotypes corresponding to B1-B3.
Oncogenes introduced into MCF-10A cells disrupt this morphogenetic process, and elicit distinct morphological phenotypes.
This inducible heterophylly of M. aquaticum leads not only to two different morphological phenotypes (see Figure 1B,C).
The morphological phenotypes included shortened flagella, increased incidence of spheromastigotes, agglutination and a novel walnut-like appearance.
In addition, the morphological phenotypes and growth duration of plants B4F1(B5F12.1B5F12), B5F1(Pi9pi9), B5F1(Sub1andB5F1 and B5F1 (Xa27xa27) were similar to that of WH421 (data not shown).
A quantitative analysis of the cell length was further performed to analyze the morphological phenotypes of the ga5dh deletion mutant (Fig. 3B and 3C).
For instance, the automated analysis of morphological phenotypes in transgenic or wild-type zebrafish would reduce workload and increase reliability in EDA [52].
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