The increased susceptibility of lkb1 larvae to Atg5 depletion during development occurred even while the yolk is not yet consumed, suggesting that even though the larvae do not show a morphological phenotype at this embryonic stage, the loss of Lkb1 appears to sensitize them to additional stress.
Interestingly, Ins2Akita-KO microglia exhibited an activated morphological phenotype at both 10 and 20 weeks age, which was accompanied by the enhanced loss of retinal ganglion cells (RGCs) and increased level of IL-1β when compared with the Ins2Akita-WT group.
Selected endpoints evaluated specific cellular-morphological phenotypes, as intersegmental vessel formation.
We next wanted to characterize the gross morphological phenotypes we observed at higher resolution to glean better insight into whether exposure to Macondo oil affected specific developmental processes during zebrafish embryogenesis.
88.21 embryos first display a morphological phenotype approximately at day 4, as they do not develop fin buds and have a swollen pericardium (Figure 1D versus wildype in Figure 1C).
The morphological phenotypes of the cell types cultured independently at early stages (passage 2) at low magnification levels (20x) and close to reaching confluence are shown in Fig. 1a c.
The morphological phenotypes induced by N-Ras-I24N at 24 hpf ranged in severity from completely disorganized embryos to mildly affected shorter embryos (Fig. 3A).
Visualization of the morphological phenotypes seen in profilin single T-DNA mutants at 2 weeks (2w), 3 weeks (3w), and 5 weeks (5w) post germination.
At day 28, three NC morphological phenotypes could be observed: (1) in clusters, with smaller vacuoles than on day 1; (2) in clusters of fibroblast-like cells; and (3) occasionally in small clusters of round cells.
However, intersubject variability in response to breathing He/O2 is large [ 3, 12, 13], resulting at least in part from variation in morphological phenotypes of disease between patients.
