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Since different BMPs are expressed on different sides of the embryo, and because no morphological landmarks exist, it was unclear where BMP signaling is active along the directive axis.
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Because Antwerp has been so physically transformed since the war, few of the old landmarks exist.
In addition, certain landmarks exist which are covered by neither of the current approaches, e.g., the lower leg landmarks of the top camera view (cf. landmarks 12 to 15 and 19 to 22 in Figure 1b).
To quantify TVC movements in four dimensions, we used morphological landmarks in the epidermis to define sagittal and frontal planes.
We used morphological landmarks (for example, the anterior commissure ventrally and the epithalamus dorsally) to maintain consistency across individuals and species.
In Figure 3, morphological landmarks are colored and evident on the skulls of Pachycephalosaurus, Dracorex and Stygimoloch.
The landmarks were based on prominent morphological landmarks such as the apical ectodermal ridge, the region where the limb attaches to the flank and to proportional distances along the main axes of the limb.
The developmental stage was determined using Downs and Davies' criteria for staging morphological landmarks of the primitive streak, neural plate and head fold [19].
Gross morphological screening was based on classical forward genetic criteria [ 8, 9] and initially focused on morphological landmarks readily visible via a dissecting microscope.
Nineteen morphological landmarks (LM; Figure 1) were plotted on each image using the software program tpsDig [ 25], which was also used to determine the Cartesian coordinates of each landmark and to establish the scale of all images.
As reliable morphological landmarks are not available for motor foot and tongue areas, these representations could only be located employing the fMRI technique.
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