A relatively large population of ciliated cells was usually observed quite early in P0 ALI-cultures (even within the first week of ALI for those layers retaining morphological integrity post-switch), while in P1 cultures, ciliated cells appeared generally at around d 30 of ALI.
The response variable used in statistical analysis was the sum of morphological integrity of capsule eggs and viability in the 24 wells with each treatment and time of exposure of the experiments.
Protocols for metabolic viability (AB), cytotoxicity (LDH release), cellular proliferation (Ki 67 expression), and morphological integrity (histopathological analysis) are described in the corresponding section.
Our results suggest that monooxygenase activity in the human small intestine is dependent on the morphological integrity of the mucosa and that in normal mucosa the enzyme rates are reduced when malassimilation is present.
The resulting structures exhibited significant differences in terms of morphological integrity, structure and mechanical properties.
Neurites contain overlapping microtubules that must be interconnected to fulfill their known functions in trafficking and morphological integrity.
The inter-relationship between hepatic intracellular pathways and the ECM is currently less studied and the elucidation of the effect of the ECMs on the metabolism is critical to identify the mechanism of in vivo hepatocellular functional and morphological integrity.
