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Secondly, an image-processing algorithm was developed to extract geometrical and morphological information for various particles.
Our work demonstrates that SHG imaging can provide morphological information for the investigation of corneal edema biophysics, and may be applied in the evaluation of advancing corneal edema in vivo.
Morphological information for each of the taxa was collected from the Smithsonian Tropical Research Institute palynological database (Jaramillo and Rueda, 2013), which contains the morphological descriptions of ∼2700 species of neotropical fossil pollen and spores.
These approaches are not of course mutually exclusive, and an ideal system would provide for both remote visualisation and the standardised packaging of morphological information for local visualisation and mark-up.
Reasons for this include the relatively simple sample preparation, the capability for retrieval of elemental and morphological information for single GSR particles, and the fact that it is a nondestructive technique.
However, the destructive nature and the challenge of obtaining morphological information for single GSR particles limit its application to GSR detection and currently SEM-EDX remains the method of choice in most circumstances.
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This study provides for the first time comprehensive micro-morphological information for three Evolvulus taxa.
The correction method could of course also make use of available morphological information from CT or MRI for very small lesions or lesions with very low contrast, if available.
Recently, quantitative phase imaging (QPI) has shown potential for extracting morphological information of individual RBCs without exogenous contrast [ 6– 8].
As well, bright field (BF) image was acquired for the morphological information of each cell by transmitted illumination using a 660nm LED light source (LEDC45, Thorlab).
The precise morphological information from MPA imaging provides an important basis for diagnosis, characterization and treatment of pathologies.
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