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After morphological identification under the compound microscope, each rope was submerged in TE buffer (pH 8.0), frozen and thawed three times.
After DNA extraction the exoskeletons of all extracted fleas were mounted (see [ 35]) and a thorough morphological identification was done under a Leica DM 3000 light microscope (Leica Microsystems, Wetzlar, Germany) using the key of [ 34].
Morphological identification was done under the taxonomic Keys by Kano and Shinonaga [ 27].
After overnight growth, yellowish colonies typical of lactobacilli were selected for morphological examination under microscope.
They were identified individually under binocular, using morphological identification keys [ 23, 24].
Ticks were all identified under light microscopy using morphological identification keys [ 20, 21].
Before the third wash, cells were inspected for morphological changes under the microscope.
Apoptosis cells morphological changes include chromatic agglutination, karyopyknosis, and nuclear fragmentation in some cells under microscope.
We confirmed the prolonged GV-arrested oocyte quality by morphological evaluation under the optical microscope and chromosome spreading.
We confirmed the long-time GV-arrested oocytes' quality by morphological evaluation under the optical microscope and chromosome spread.
The MO-injected or uninjected embryos were grown at 28.5°C and observed for morphological changes under a stereoscopic microscope at 23.5 to 28.5 hpf.
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