Morphological dominant expression was tested with an ANOVA in which the variable was the Euclidean distance of each hybrid male to the centroid of each parental species (mean parental shape) with Cross and Parents as fixed factors.
Levels of hVEGF-A165 expression were moderate and morphological changes were found mainly in the liver, showing typical signs of active angiogenesis.
Morphological changes caused by EGFP-MVaf1 expression were characterized by fluorescence microscopy, in Z-stack acquisition mode.
The authors [ 3] report that the miRNA-based approach can reprogram up to 10% of the input cells, although this could be an overestimation as only morphological criteria, and not pluripotency marker expression, were used to quantify the efficiency of reprogramming of human fibroblasts.
Regions of differential expression were contiguous and showed similar morphological phenotype in all cases.
In agreement with the observed morphological changes, SKP2 expression was restored by WT-STAT3 in BIS-depleted cells, which led to a decrease in p27 protein levels; there was no effect from either inactive mutant.
Morphological changes and protein expression are not conclusive in the stem cell research field and, therefore, the plasticity of stem cells requires careful validation.
While cell division is intuitively simple and clear from a morphological perspective, a mathematical expression is necessary to provide a usable description of propagation of an individual cell during a clone lifetime.
Stable expression of ICSBP induced EMT-like morphological changes, and Snail expression was increased by ICSBP expression in both the cell lines.
Inflammatory reactivity in microglia, based on morphological changes and cytokine expression, was investigated in G1 mTerc−/− and G3 mTerc−/− mice during intraperitoneal injection of lipopolysaccharide.
