Moreover the morphological experiments utilizing FE-SEM, supportheheterogeneityity present in the peptides.
For morphological experiments, dehydration at −90°C lasted at least 48 h in acetone containing 2% osmium tetroxide, 0.25% uranyl acetate, 0.25% glutaraldehyde and 1% H2O.
We performed further biochemical and morphological experiments to verify these results in transcriptome.
The morphological experiments show that Golgi vesicles are depleted (80%) of albumin while Golgi intercisternal tubules appear to contain albumin at similar levels as those seen in cisternae.
All the morphological experiments published in a selection of primary papers from the planarian literature have been curated and introduced in the freely-available database.
For statistical analyses of data obtained from the electrophysiological and morphological experiments, Student's t test was employed unless otherwise mentioned.
I estimate that the electrophysiological and morphological experiments necessary to test the prediction would take my lab the better part of a year to complete.
Notice that to be convincing the morphological experiments proposed by the reviewer would have to be preceded by electrophysiological experiments confirming that the 5X5HT-1X5HT-Aniso-3X5HT treatment produces LTF and that, as we would expect, 3X5HT alone does not.
Prior to the SEM morphological experiments the hydrogel samples were lyophilized, frozen in liquid nitrogen, and then broken in order to avoid the deformations caused by the fracture of the samples.
L.A., A.G., H.B., and M.D., designed the experiments, A.S. and A.G. established the photo-stimulation setup, H.B. and A.G. performed physiological experiments, V.P. performed morphological experiments, H.B., B.H., A.G., and R.A.M. analyzed the data; L.A., H.B., A.G., and R.A.M. wrote the manuscript with the contribution of all authors.
