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The biological characterizations of the HAM membrane were performed by cell proliferation and their morphological examinations using rat cardiac fibroblasts (RCF), NIH3T3 fibroblasts, A431 keratinocytes, L6 myocytes and HepG2 hepatocytes.
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Morphological examination using scanning electron microphotography demonstrated spherical particles with a main diameter of 15 30 μm.
Gross morphological examination using scanning electron microscopy indicated good integration of host tissue with scaffold/cell constructs and extensive tissue infiltration into the scaffold interior.
Pour plating was also done with dilutions of 1∶107 and 1∶108 and the submerged colonies were selected for morphological examination using Gram staining.
Apoptosis was evaluated by morphological examination using DAPI staining.
Species identification of collected material was determined by morphological examination using a taxonomic key to the United States species of Drosophila [ 66].
Electrophysiological studies and morphological examination using both light and electron microscopy have revealed no evidence of retinal toxicity after perfluorophenanthrene intravitreal placement for periods up to 48 hours [ 47, 48].
The placentas were conventionally diagnosed as accreta (superficially implanted), increta (within the myometrium), and percreta (through the myometrium) by morphological examination using clear evidence of loss of decidua and the degree of myometrial adhesion as criteria.
AgNPs were shown to promote apoptosis as seen in the nuclear morphological examination study using propidium iodide staining and DNA fragmentation by single cell gel electrophoresis technique.
Morphological examination, performed using endogenous tissue fluorescence, discriminated adenomatous and adenocarcinoma tissues from normal mucosa in terms of cellular asymmetry and nucleus-to-cytoplasm ratio.
Non-adherent cells were removed by washing the wells three times with RPMI, and the remaining adherent cells (>97% macrophages as assessed by morphological examination) were used for experiments.
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