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Maximum likelihood analysis, using DNA sequence data from the internal transcribed spacer region (ITS) of the ribosomal DNA repeat, DNA sequencing of the elongation factor 1-α gene, cultural pairing studies and morphological examination were undertaken to confirm the novelty of Armillaria sp. nov. as a distinct species.
The vessel segments used for morphological examination were cleaned of connective tissues and the endothelial cell layer was left intact.
In the assessment of mast cell occurrence, all mast cells occurring in the whole half bladder segment (the half separated for morphological examination) were counted except for those in the serosa.
Non-adherent cells were removed by washing the wells three times with RPMI, and the remaining adherent cells (>97% macrophages as assessed by morphological examination) were used for experiments.
The first two series of morphological examination were done on two control groups of animals which underwent an occlusion of middle cerebral artery and were decapitated after 6 and 12 days, respectively.
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However, the diagnosis of cancer based on morphological examination is not always accurate, as the morphology of malignant cells often resembles the common ones and it depends on the physician' skill and knowledge.
The morphological examination was characterized by transmission electron microscopy (TEM), and the resultant particle sizes were analyzed by the ImagingJ software.
Morphological examination was performed "blind" to the treatment.
Morphological examination was conducted under a light microscope (BX-FM: Olympus Corp ,Tokyo, Japan).
Sperm morphological examination was performed using formaldehyde-fixed samples under phase-contrast microscopy (1000×).
Morphological examination was performed by electron microscopy and hematoxylin-eosin (H&E) staining.
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