In the morphological examination, the LDS interneuron was stained with Lucifer yellow CH, fixed in 10% formalin, dehydrated through ascending ethanol series, and cleared in methylsalicylate.
For morphological examination, the H&E staining was carried out according to routine histological practice.
For standard morphological examination, the glutaraldehyde-fixed fragments were washed and then postfixed in 1% osmium tetroxide for 2 hours.
In addition to morphological examination, the wildlife pathologist may also utilise various tests, such as isolation of the infectious agent, detection of antigen in tissues by immunohistochemistry or polymerase chain reaction [ 2, 3].
For morphological examination, the cells were cytocentrifuged onto a microscope slide using a Cytospin (Shandon Southern Instrument Inc., Sewelicky, PA, USA), stained with Liu's stain, and observed under an upright microscope (Olympus, Tokyo, Japan) at a magnification of 1,000x.
As identified in the morphological examination, the sequencing of the amplicons confirmed the presence of parasites of the genera Mesocestoides (identity ranging from 87% and 92% to M. lineatus DNA, accession number L49450) and of T. taeniaeformis (identity ranging from 98% and 99%, accession number EU219537) explaining thus the positive results of the first PCR.
The morphological examination of the cells after these doses confirmed the NR findings.
The morphological examination of the conidia projected from those aphid samples showed the presence of both P. neoaphidis and E. planchoniana.
In addition to the morphological examination of the cross-section preparation and SEM EDS analysis, FTIR spectroscopy supported the conclusion drawn as spectra of bone black, ivory black and carbon black were compared with one obtained from the black sample.
The morphological examination of the NPs was performed by scanning electron microscopy (SEM; Carl Zeiss SMT, Oberkochen, Germany) [15].
A total of over 5000 herbarium specimens were included in the morphological examination and the phenetical analysis included 29 generative and vegetative characters.
